HGF and BMP-7 ameliorate high glucose-induced epithelial-to-mesenchymal transition of peritoneal mesothelium.

epitome\nOver time, peritoneal dialysis results in functional and structural alterations of the peritoneal membrane, besides the underlying mechanisms and whether these changes ar reversible are not completely understood. Here, we studied the effectuate of advanced levels of glucose, which are found in the dialysate, on piece peritoneal mesothelial cells (HPMCs). We found that advanced densenesss of glucose possessd epithelial-to-mesenchymal transition (EMT) of HPMC, suggested by decrease expression of E-cadherin and increase expression of alpha-smooth muscle actin, fibronectin, and oddball I collagen and by change magnitude cell migration. Normalization of glucose concentration on day 2 reversed the phenotypic transformation, but the changes were irreversible after 7 d of stimulation with gritty glucose. In addition, exposure of HPMC to high glucose resulted in a decrease expression of the antifibrotic cytokines, hepatocyte growth doer (HGF) and bone morphogenic prot ein 7 (BMP-7). exogenic treatment with HGF resulted in a dosage-dependent prevention of high glucose-induced EMT. some(prenominal) BMP-7 peptide and gene transfection with an adenoviral vector of BMP-7 likewise protected HPMCs from EMT. Furthermore, adenoviral BMP-7 transfection decreased peritoneal EMT and ameliorated peritoneal thickening in an animal(prenominal) model of peritoneal dialysis. In summary, high concentrations of glucose induce a reversible EMT of HPMCs, associated with decreased production of HGF and BMP-7. Treatment of HPMCs with HGF or BMP-7 blocks high glucose-induced EMT, and BMP-7 ameliorates peritonealfibrosis in an animal model of peritoneal dialysis.If you pauperization to get a wide-cut essay, order it on our website:

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